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柴利军
时间:2017-09-05

柴利军   博士、副教授、硕士生导师

华中农业大学“狮山硕彦计划” 青年英才A类人才。1983年7月生,籍贯陕西府谷。2005年7月本科毕业于华中农业大学园艺系;2005-2012年,华中农业大学攻读硕士、博士学位;2012年6月毕业,获博士学位并留校任教。2015年晋升为副教授。2011-2012年,英国伯明翰大学生物技术系开展合作研究。

Tel: 027-87287593 (O);Email: chailijun@mail.hzau.edu.cn


国内外学术兼职:

担任国际SCI刊物《3 Biotech》副主编,Frontiers in Plant Science审稿编辑(review editor);Plant and Cell Physiology、Molecular Breeding、Gene、Plos one、Scientia Horticulturae、Molecular Biology Reports、National Academy Science Letters、International Journal of Molecular Sciences、园艺学报、果树学报等期刊审稿专家;中国柑橘学会理事;国家自然科学基金通讯评审人;国际植物有性生殖学会(ICSPR)会员。

 

研究方向

柑橘种质资源与遗传改良、柑橘生殖生物学研究

 

重点内容

1)针对我国野生、半野生及农家柑橘资源保护、评价及开发利用不足,部分珍贵资源面临消失灭绝等现实问题,系统开展芸香科植物种质资源调查、收集、保存,遗传多样性检测,亲缘关系鉴定及性状评价;2)针对生产中由于自交不亲和性状引起的座果率低及果实品质差异等问题,利用分子生物学、生物化学、细胞生物学等手段结合组学方法,系统开展自交不亲和性状解析与控制自交不亲和性状雌/雄蕊关键决定因子发掘与鉴定。

 

主持或承担的科研项目:

1. 芸香科植物自交不亲和分子机制研究。主持,编号:2018YFD1000107,国家重点研发计划子课题,2018.07-2022.12。

2. 花粉SLF基因在沙田柚自交不亲和反应中的作用及机制研究。主持,编号:31772259,国家自然科学基金面上项目,2018.1~2022.12。

3. 芸香科植物自交不亲和S位点进化机制研究。主持,编号:2662019PY044,华中农业大学自主科技创新基金,2019.1~2021.12。

4. SRK基因在沙田柚自交不亲和反应中的功能解析。主持,编号:31301760,国家自然科学基金青年基金,2014.1~2016.12。

5. 云南野生半野生柑橘种质资源收集、保存及遗传多样性评价。参与,编号:31460507,国家自然科学基金地区基金,2015.1~2018.12。

6. 控制沙田柚自交不亲和性候选基因的分离克隆及功能鉴定。主持,编号:2013QC037,青年教师科技创新专项,2013.01-2014.12。

7. 沙田柚自交不亲和分子机理研究。主持,编号:0900206096,博士科研启动费。


发表论文(* 通讯作者) 

1. Liang Mei, Cao Zonghong, Zhu Andan, Liu Yuanlong, Tao Mengqin, Yang Huayan, Xu Jr Qiang, Wang Shaohua, Liu Junjie, Li Yongping, Chen Chuanwu, Xie Zongzhou, Deng Chongling, Ye Junli, Guo Wenwu, Xu Qiang, Xia Rui, Larkin RM, Deng Xiuxin, Bosch Maurice, Franklin-Tong Vernonica E, Chai Lijun* (2020) Evolution of self-compatibility by a mutant Sm-RNase in citrus. Nature Plants 6:131-142.

2.韦壮敏,李蒙蒙,杨晓明,袁晓慧,叶俊丽,谢宗周,邓秀新,柴利军*(2020). 无核酸橙资源‘抛橘’的遗传鉴定。园艺学报472):345-354.

3. 李昂,戴冬,赵立,韦壮敏,丁德宽,袁晓慧,叶俊丽,谢宗周,邓秀新,柴利军* (2019) 基于胚抢救技术的枳雀×枳杂交群体创建及鉴定。果树学报364):430-437.

4. 李 航,杨晓明,丁德宽,王小丽,谢宗周,叶俊丽,彭抒昂,邓秀新,柴利军* (2018) 基于cpSSRnSSR标记的地方柑橘资源“枳雀”亲本分析。果树学报3510):265-274.

5. Zhang Siqi, Liang Mei, Wang Nan, Xu Qiang, Deng Xiuxin, Chai Lijun* (2018) Reproduction in woody perennial Citrus: an update on nucellar embryony and self-incompatibility. Plant Reproduction 31:43-57.

6. Chai Lijun, Tudor RL, Poulter NS, Wilkins KA, Eaves DJ, Franklin FCH, Franklin-Tong VE* (2017) MAP Kinase PrMPK9-1 contributes to the Self-Incompatibility Response. Plant Physiology174 (2) 1226-1237.

7. Liang Mei, Yang Wei, Su Shiying, Fu Lili, Yi Hualin, Chuanwu Chen, Deng Xiuxin, Chai Lijun* (2017) Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibilityof citrus. Molecular Genetics and Genomics292: 325-341.

8. Wang Xiaohua, Yin Wu, Wu Juxun, Chai Lijun*, Yi Hualin* (2016) Effects of exogenous abscisic acid on the expression of citrus fruit ripening-related genes and fruit ripening. Scientia Horticulturae 201:175-183.

9. Liang Mei, Yang Xiaoming, Li Hang, Su Shiying, Yi Hualin, Chai Lijun*, Deng Xiuxin (2015) De Novo Transcriptome Assembly of Pummelo and Molecular Marker Development. PLoS ONE 10(3):e0120615.

10. Li Hang, Yang Xiaoming, Zhu Lianshu, Yi Hualin, Chai Lijun*, Deng Xiuxin (2015) Parentage analysis of natural citrus hybrid ‘Zhelong Zhoupigan’ based on nuclear and chloroplast SSR markers. Scientia Horticulturae 186:24-30.

11. Zhang Ya-Jian, Wang Xing-Jian, Wu Ju-Xun, Chen Shan-Yan, Chen Hong, Chai Li-Jun*, Yi Hua-Lin* (2014) Comparative Transcriptome Analyses between a Spontaneous Late-Ripening Sweet Orange Mutant and Its Wild Type Suggest the Functions of ABA, Sucrose and JA during Citrus Fruit Ripening. PLoS ONE 9(12):e116056.

12. Wu Juxun, Su Shiying, Fu Lili, Zhang Yajian, Chai Lijun*, Yi Hualin* (2014) Selection of reliable reference genes for gene expression studies using quantitative real-time PCR in navel orange fruit development and pummelo floral organs. Scientia Horticulturae. 176: 180-188.

13. Chai Lijun, Ge Xiaoxia, Kumar Biswas Manosh, Xu Qiang, Deng Xiuxin* (2011) Self-sterility in the mutant ‘Zigui shatian’ pummelo (Citrus grandis Osbeck) is due to abnormal post-zygotic embryo development and not self-incompatibility. Plant Cell Tissue and Organ Culture 104:1-11.

14. Chai Lijun, Ge Xiaoxia, Xu Qiang, Deng Xiuxin* (2011) CgSL2, an S-like RNase gene in ‘Zigui shatian’ pummelo (Citrus grandis Osbeck), is involved in ovary senescence. Molecular Biology Reports 38:1-8.

15. Chai Lijun, Kumar Biswas Manosh, Ge Xiaoxia, Deng Xiuxin* (2010) Isolation, characterization, and expression analysis of an SKP1-like gene from ‘Shatian’ Pummelo (Citrus grandis Osbeck). Plant Molecular Biology Reporter 28:569-577.

16. Chai Lijun, Ge Xiaoxia, Kumar Biswas Manosh, Deng Xiuxin* (2011) Molecular analysis and expression of a floral organ-relative F-box gene isolated from 'Zigui shatian' pummelo (Citrus grandis Osbeck). Molecular Biology Reports 38:4429-4436.

17. Chai Lijun, Kumar Biswas Manosh, Yi Hualin, Guo Wenwu, Deng Xiuxin* (2013) Transferability, polymorphism and effectiveness for genetic mapping of the Pummelo (Citrus grandis Osbeck) EST-SSR markers. Scientia Horticulturae 155:85-91.

18. Kumar Biswas Manosh, Chai Lijun (Co-first author), Xu Qiang, Deng Xiuxin* (2012) Generation, functional analysis and utility of C. grandis EST from a flower-derived cDNA library. Molecular Biology Reports 39: 7221-7235.

19. de Graaf BHJ, Vatovec S, Andrés Juárez-Díaz J, Chai Lijun, Kooblal K, Katie A. Wilkins KA, Zou Huawen, Forbes T, Franklin FCH, Franklin-Tong VE (2012) The Papaver Self-Incompatibility Pollen S-Determinant, PrpS, Functions in Arabidopsis thaliana. Current Biology 22:1-6.

 

申请专利

1. 柴利军,梁梅,曹宗洪,邓秀新。一种决定柑橘柚植物自交不亲和性的基因及其应用。申请号:201811165648.9.

2. 柴利军,杨华艳,邓秀新。一种鉴定柑橘植株根腐病抗性的方法。申请号:201811165642.1.

3. 柴利军,梁梅,曹宗洪,邓秀新。离体验证柑橘柚类S-RNase自交不亲和功能的方法。申请号:201811195445.4.

4. 柴利军,梁梅,曹宗洪,邓秀新。基于S基因型的为柑橘柚鉴定授粉供体的试剂盒及方法。申请号:201811165641.7.

5. 柴利军,曹宗洪,梁梅,邓秀新。利用InDel标记鉴定柑橘柚类植物杂交子代的试剂盒及方法。申请号:201811242378.7.

6. 柴利军,胡健兵,梁梅,邓秀新。一种快速鉴定柑橘自交亲和性的分子标记及其应用。申请号:201910506486.9.

7. 柴利军,蒋靖东,韦壮敏,邓秀新。一种适合活体快速鉴定柑橘自交亲和与否的方法。申请号:201910509166.9.

 

学术交流口头/墙报汇报:

1. 柴利军,.沙田柚自交不亲和分子机制研究。广西特色作物研究院学术交流,中国广西,桂林,2018-8-2.

2. 柴利军Identification of female component of self-incompatibility inCitrus grandisOsbeck.(墙报)第25届国际植物有性生殖会议,日本,岐阜,2018/06/11-2018/06/16.

3. 柴利军Exploiting Wild and Dooryard Citrus Germplasm for Genetics and Genomics Research. 第十三届国际柑橘学大会,巴西,伊瓜苏,2016/09/17-2016/09/27.

4. 柴利军Identification of stigma/pollen specifically expressed genes related to the self-incompatibility in‘Shatian’pummelo (Citrus grandisOsbeck) .第十三届国际柑橘学大会,巴西,伊瓜苏,2016/09/17-2016/09/27.

5. 柴利军,基于cpSSR和EST-SSR标记的‘皱皮黄果’遗传背景研究。中国柑橘学会2014学术年会,中国福建,平和,2014-10-16.

6. 柴利军,Searching for candidate proteins conferring self-incompatibility of ‘Shatian’ pummelo (Citrus grandisOsbeck) based on comparative proteomic (Digital posters presentation).第29届国际园艺学大会,澳大利亚,布里斯班,2014-08-21.

7. 柴利军,适合柑橘遗传多样性分析和遗传连锁图谱构建的柚EST-SSR标记开发。中国园艺学会2012年学术年会,中国陕西,杨凌,2012-10-20.


信息更新于2020年2月