伍小萌 博士/副教授
通讯地址:430070湖北省武汉市华中农业大学
园艺植物生物学教育部重点实验室
联系电话:027-87287393
Email:wuxm@mail.hzau.edu.cn
教育及工作经历
2018/01 –,华中农业大学,园艺林学学院,副教授
2015/01 – 2017/12,华中农业大学,园艺林学学院,讲师
2013/04 – 2014/04,美国加州大学洛杉矶分校(UCLA)分子细胞发育生物学系(MCDB),博士生联合培养,导师:Bob Goldberg教授(美国科学院院士)
2006/09 – 2014/12,华中农业大学,园艺林学学院,果树学博士,导师:郭文武教授
2002/09 – 2006/06,华中农业大学,园艺林学学院园艺专业,农学学士
主讲课程:本科生《试验设计与统计分析》、《园艺植物研究法》、《园艺概论》慕课,研究生《学术道德与科技写作》
承担科研项目:先后主持国家自然科学基金(面上2项,青年1项)、国家重点研发计划课题(1项)等多个科研项目。
研究方向:柑橘无性生殖机理与生物技术改良
1)柑橘无性胚发生机理及利用:无性胚发生,包括离体(in vitro)体细胞胚发生(somatic embryogenesis)和活体(in vivo)珠心多胚(nucellar poly-embryony)。体细胞胚发生是以愈伤组织为外植体的柑橘病毒脱除与品种提纯复壮、原生质体融合、基因编辑等生物技术改良的必要再生步骤。珠心多胚是典型的孢子体无融合生殖现象,被用于繁育整齐一致的砧木和固定优良品种的种性;但珠心胚干扰合子胚发育,又是柑橘常规杂交育种的主要障碍。拟发掘无性胚发生的关键基因并解析调控路径,揭示无性胚发生机理,建立优化原生质体基因编辑等生物技术,为柑橘繁育和遗传改良效率提升提供理论基础和技术体系。
2)柑橘雄性不育机理及利用:雄性不育(Male sterility)表现为雄蕊缺失、花药发育异常和花粉败育,隔离种植果实无核,被广泛用于柑橘无核改良。拟利用课题组自主创制的雄性不育胞质杂种及其遗传分离群体,发掘控制雄蕊发育和影响花粉育性的关键因子,揭示柑橘细胞质雄性不育(CMS)机理,培 育兼具多胚和雄性不育/果实无核的柑橘新种质。
发表论著(*通讯作者,#共同第1作者)
1)Chen M, Lin JY,Wu XM, Apuya NR, Henry KF, Le BH, Bui AQ, Pelletier JM, Cokus S, Pellegrini M, Harada JJ, Goldberg RB (2021) Comparative analysis of embryo proper and suspensor transcriptomes in plant embryos with different morphologies.Proc. Natl. Acad. Sci. U.S.A.118: e2024704118(激光捕获精细取样结合转录组,解析不同植物球形胚时期胚体和胚柄的基因表达及功能特征)
2) Xu YT, Jia HH,Wu XM, Koltunow AMG, Deng XX, Xu Q (2021) Regulation of nucellar embryony, a mode of sporophytic apomixis inCitrusresembling somatic embryogenesis.Current Opinion in Plant Biology59:101984(柑橘珠心多胚性调控评述)
3) Zhang S, Yin ZP,Wu XM*, Li CC, Xie KD, Deng XX, Grosser JW, Guo WW* (2020) Assembly of Satsuma mandarin mitochondrial genome and identification of cytoplasmic male sterility–specific ORFs in a somatic cybrid of pummelo.Tree Genetics and Genomes, 16: 84(组装柑橘线粒体基因组,利用自主创制的雄性不育胞质杂种柚鉴定CMS特异ORFs)
4) Wang R, Fang YN,Wu XM*, Qing M, Li CC, Xie KD, Deng XX, Guo WW* (2020) miR399-CsUBC24 module regulates the reproductive development and male fertility in citrus.Plant Physiology, 183: 1681–1695(*Co-Senior Author,以柑橘雄性不育胞质杂种为材料,揭示miR399与靶基因CsUBC24调控柑橘花发育和雄性不育的新功能)
5) Liu Z, Ge XX,Wu XM*, Xu Q, Atkinson RG, Guo WW (2020) Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis.BMC Genomics21: 305(鉴定柑橘B3超家族基因,筛选体细胞胚发生相关基因CsARF19)
6) Fang YN, Yang XM, Jiang N,Wu XM*, Guo WW (2020) Genome-wide identification and expression profiles of phased siRNAs in a male-sterile somatic cybrid pummelo (Citrus grandis).Tree Genetics & Genomes, 16: 46(柚胞质杂种雄性不育相关phasiRNA筛选)
7) Liu Z, Ge XX, Qiu WM, Long JM, Jia HH, Yang W, Dutt M,Wu XM*, Guo WW* (2018) Overexpression of theCsFUS3gene encoding a B3 transcription factor promotes somatic embryogenesis inCitrus.Plant Science277: 121-131(B3转录因子CsFUS3调控柑橘体细胞胚发生功能解析)
8) Long JM, Liu CY, Feng MQ, Liu Y,Wu XM*, Guo WW* (2018) miR156-SPLmodules regulate induction of somatic embryogenesis in citrus callus.Journal of Experimental Botany69: 2979-2993(miR156与靶基因CsSPLs调控柑橘体细胞胚发生的功能解析)
9) Liu MY#,Wu XM#*, Long JM, Guo WW (2017) Genomic characterization of miR156 andSQUAMOSA promoter binding protein-likegenes in sweet orange (Citrus sinensis).Plant Cell Tissue And Organ Culture130: 103-116(柑橘miR156家族及其CsSPL靶基因鉴定与分析)
10) Long JM, Liu Z,Wu XM*, Fang YN, Jia HH, Xie ZZ, Deng XX, Guo WW*(2016) Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis.Journal of Experimental Botany67: 5743–5756(胚珠小RNA和转录组发掘柑橘珠心胚起始发生的关键基因和miRNA)
11) Wu XM, Kou SJ, Liu YL, Fang YN,Xu Q, Guo WW* (2015)Genomewide analysis of small RNAs in nonembryogenic and embryogenic tissues of citrus: microRNA- and siRNA- mediated transcript cleavage involved in somatic embryogenesis.Plant Biotechnology Journal13: 383-394(高通量组学发掘柑橘体细胞胚发生相关小RNA)
12) Kou SJ#,Wu XM#, Liu Z, Liu YL, Xu Q, Guo WW* (2012)Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues.Plant Cell Reports31: 2151-2163(柑橘体细胞胚发生系统的miRNA定量RT-PCR内参基因筛选)
13) Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011)Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange.Planta233: 495-505(植物保守miRNA在柑橘体细胞胚发生过程中的时空表达模式解析)
14) Wu XM, Liu MY, Xu Q, Guo WW* (2011) Identification and characterization of microRNAs from citrus expressed sequence tags.Tree Genetics & Genomes7: 117-133(基于EST数据库鉴定柑橘miRNAs)
参加的部分国际学术会议及口头汇报(*汇报人)
1. Wu XM*(2020) The miRNAs and genes associated with asexual embryogenesis in citrus.第七届国际园艺研究大会(7.1-7.30)网络会议,育种专题主持、青年学者论坛口头报告
2. Jia HH, Yin ZP, Xu YT, Tan XY,Wu XM*, Guo WW (2019) Understanding citrus nucellar poly-embryony.第六届国际园艺研究大会墙报口头展示并获优秀墙报一等奖,意大利威尼斯9.30-10.5
3. Wu XM*, Long JM, Liu Z, Jia HH, Feng MQ, Guo WW (2017)Understanding in vitro somatic embryogenesis and nucellar embryony of citrus.第二届HZAU-PFR双边果树科学研讨会,新西兰奥克兰3.12-14
4. Wu XM*(2016)Understandingin vitrosomatic embryogenesis and nucellar embryony of citrus.第13届国际柑橘学大会,荣获国际柑橘学会“青年学者”荣誉奖(Young Scientist Award Honorable Mention),巴西伊瓜苏9.18-23
5. Wu XM*, Long JM, Kou SJ, Guo WW(2014)High-throughput sequencing uncovers miRNAs and siRNAs involved in citrus somatic embryogenesis.第22届国际植物与动物基因组大会—柑橘基因组研讨会,美国加州圣地亚哥1.10-15
6. Wu XM*, Chen M, Henry KF, Goldberg RB(2013)What is the function of the suspensor – a comparative genomics approach.第21届种子年会,美国洛杉矶11.15-17
7. Wu XM*, Liu MY, Xu Q, Deng XX, Guo WW(2012)Exploring miRNA-targets modulation in citrus somatic embryogenesis by high-throughput small RNA and degradome sequencing.第12届国际柑橘学大会,西班牙巴伦西亚11.18-23
信息更新于2021年3月